Table 3.
Primer list used for Wolbachia detection.
Gene | Hypothetical product | Primer name and sequences (5′–3′) | Product size | Tm (°C) | References |
---|---|---|---|---|---|
16S rRNA | Ribosomal RNA 16S |
16S 281F: CTATAGCTGATCTGAGAGGAT 16S 1372R: YGCTTCGAGTGAAACCAATTC |
1,100 | 55 | Wang et al.4 |
16S rRNA | Ribosomal RNA 16S |
WspecF: CAT ACC TAT TCG AAG GGA TAG WspecR: AGC TTC GAG TGA AAC CAA TTC |
440 | 52 | Werren and Windsor43 |
wsp | Outer surface protein |
Wsp81F: TGGTCCAATAAGTGATGAAGAAAC Wsp691R: AAAAATTAA ACGCTACTCCA |
546 | 52 | Zhou and Rousset40 |
groEL | Chaperonin GroEL |
GroELF: GGTGAGCAGTTRCARSAAGC GroELR: TARCCRCGRTCAAAYTGCATRCCA |
491 | 55 | Wang et al.4 |
ftsZ | Cell division protein |
FtsZF: GTTGGYAAAGGTGCAGCAGAAGA FtsZR: CGYACYCATTTKGCTGCAGMATCAA |
524 | 53 | Wang et al.4 |
groEL | Chaperonin GroEL |
groEL F1: GGTGAGCAGTTGCAAGAAGC groEL R1: AGRTCTTCCATYTTRATTCC |
491 | 55 | Casiraghi et al.41 |
gltA | Citrate synthase |
gltAF1: TACGATCCAGGGTTTGTTTCTAC gltAR1: CTCATTAGCTCCACCGTGTG |
659 | 56 | Casiraghi et al.41 |
gatB | Glutamyl-tRNA(Gln) amidotransferase, subunit B |
GatB F1: GAKTTAAAYCGYGCAGGBGTT GatB R1: TGGYAAYTCRGGYAAAGATGA |
497 | 54 | Paraskevopoulos et al.42 |
coxA | Cytochrome coxidase, subunit I |
CoxA-F1: TTGGRGCRATYAACTTTATAG CoxA-R1: CTAAAGACTTTKACRCCAGT |
488 | 55 | Paraskevopoulos et al.42 |
hcpA | Conserved hypothetical protein |
HcpA-F1: GAAATARCAGTTGCTGCAAA HcpA-R1: GAAAGTYRAGCAAGYTCTG |
515 | 53 | Paraskevopoulos et al.42 |
fbpA | Fructose-bisphosphate aldolase |
FbpA-F1: GCTGCTCCRCTTGGYWTGAT FbpA-R1: CCRCCAGARAAAAYYACTATTC |
509 | 59 | Paraskevopoulos et al.42 |