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. 2020 Jul 21;10:12025. doi: 10.1038/s41598-020-68925-z

Table 3.

Primer list used for Wolbachia detection.

Gene Hypothetical product Primer name and sequences (5′–3′) Product size Tm (°C) References
16S rRNA Ribosomal RNA 16S

16S 281F: CTATAGCTGATCTGAGAGGAT

16S 1372R: YGCTTCGAGTGAAACCAATTC

1,100 55 Wang et al.4
16S rRNA Ribosomal RNA 16S

WspecF: CAT ACC TAT TCG AAG GGA TAG

WspecR: AGC TTC GAG TGA AAC CAA TTC

440 52 Werren and Windsor43
wsp Outer surface protein

Wsp81F: TGGTCCAATAAGTGATGAAGAAAC

Wsp691R: AAAAATTAA ACGCTACTCCA

546 52 Zhou and Rousset40
groEL Chaperonin GroEL

GroELF: GGTGAGCAGTTRCARSAAGC

GroELR: TARCCRCGRTCAAAYTGCATRCCA

491 55 Wang et al.4
ftsZ Cell division protein

FtsZF: GTTGGYAAAGGTGCAGCAGAAGA

FtsZR: CGYACYCATTTKGCTGCAGMATCAA

524 53 Wang et al.4
groEL Chaperonin GroEL

groEL F1: GGTGAGCAGTTGCAAGAAGC

groEL R1: AGRTCTTCCATYTTRATTCC

491 55 Casiraghi et al.41
gltA Citrate synthase

gltAF1: TACGATCCAGGGTTTGTTTCTAC

gltAR1: CTCATTAGCTCCACCGTGTG

659 56 Casiraghi et al.41
gatB Glutamyl-tRNA(Gln) amidotransferase, subunit B

GatB F1: GAKTTAAAYCGYGCAGGBGTT

GatB R1: TGGYAAYTCRGGYAAAGATGA

497 54 Paraskevopoulos et al.42
coxA Cytochrome coxidase, subunit I

CoxA-F1: TTGGRGCRATYAACTTTATAG

CoxA-R1: CTAAAGACTTTKACRCCAGT

488 55 Paraskevopoulos et al.42
hcpA Conserved hypothetical protein

HcpA-F1: GAAATARCAGTTGCTGCAAA

HcpA-R1: GAAAGTYRAGCAAGYTCTG

515 53 Paraskevopoulos et al.42
fbpA Fructose-bisphosphate aldolase

FbpA-F1: GCTGCTCCRCTTGGYWTGAT

FbpA-R1: CCRCCAGARAAAAYYACTATTC

509 59 Paraskevopoulos et al.42