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Time- and ABC transporter-dependent effects of LY294002 on the size of the Rh123low pool. OCR_OCMM1 (A) and OCR_OCMM2 (B) sphere cells were treated with 10 μM of LY294002 (+) or growth medium (−) for different durations during the Rh123 dye exclusion assay (pretreatment-30 min; labeling-20 min; and exclusion-60 min) (n = 8). A final concentration of 50 μM verapamil (V) was added as a control where indicated; (0) indicates no exclusion. *P ≤ 0.05; ***P ≤ 0.005; and ****P ≤ 0.001.
