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. 2020 Jul 21;10:12076. doi: 10.1038/s41598-020-68941-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative images of apoptotic cells in the hemolymph of adult male G. coquereliana obtained using fluorescence microscopy at different magnifications (a,b—× 40; c,d—× 100). Hemocytes from control insects (a,c) and cold-stressed insects (b,d) were stained with SR-VAD-FMK to analyze caspase activity (red), while DAPI was used for DNA staining (blue), and Oregon Green 488 phalloidin was used to visualize the F-actin cytoskeleton (green). Yellow arrows indicate apoptotic cells. The bars show a 20 µm scale.