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. 2020 Jul 21;10:12031. doi: 10.1038/s41598-020-68721-9

Figure 1.

Figure 1

The HepG2 human hepatoma cell line displays aberrant insulin signaling compared to murine primary hepatocytes. (a) Immunoblot analysis of insulin signaling in primary mouse hepatocytes infected with a control adenovirus expressing the GFP or a virus expressing the negative dominant RAS mutant H-RAS17N and stimulated with 10 nM insulin for 8 min. (b) Quantification of blots in (a). (c) Immunoblot analysis of insulin signaling and insulin receptor phosphorylation performed as in (a) but using the HepG2 cell line. (d) Quantification of blots in (c). n = 3 biological replicates (mice) for primary hepatocytes and 3 independent experiments for HepG2. Data are represented as mean ± SEM.