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. 2020 Jul 21;10:12031. doi: 10.1038/s41598-020-68721-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hepatoma cell lines display reduced fatty acid synthase expression and a distinct protein electrophoresis pattern. (a) Immunoblot analysis of FAS on primary hepatocytes, Hepa 1–6, McARH7777, and HepG2. (b) Quantification of blots in (a). (c) Coomassie staining of a protein electrophoresis of extracts from human primary hepatocytes, murine primary hepatocytes, Hepa 1–6, McARH7777, and HepG2. n = 9 for a and b; n = 2–3 for c. n indicates biological replicates for primary hepatocytes, technical replicates for human hepatocytes, and independent experiments for cell lines. Data are represented as mean ± SEM.