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. 2020 Jul 21;10:12109. doi: 10.1038/s41598-020-68694-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth advantage at the population level under axenic conditions. A. castellanii cells were allowed to adhere and replicate for 24 h without agitation. The multiplication factors represent the number of amoebae at 48 h divided by the number of amoebae at 24 h (see also Fig. 1 for workflow). Data are generated from three biological independent experiments, which were run using technical duplicates. Error bars ± SEM. (*) p < 0.05; (**) p < 0.01 and (ns) p > 0.05.