Extended Data Fig. 2: Sedimentation equilibrium analysis of mini-Ins.
Sedimentation equilibrium analysis of mini-Ins was performed at 35,000 rpm with the best fit (curves) to a single species of apparent mass 5080 ± 45 Da. The molecular weight of mini-Ins is 5067. Detailed procedure can be found in reference: Menting et al. (2016). A minimized human insulin-receptor-binding motif revealed in a Conus geographus venom insulin. Nature Structural & Molecular Biology, 23(10), 916. Source data are available with the paper online.