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. 2020 Jul 21;11:3652. doi: 10.1038/s41467-020-17433-9

Fig. 4. Sphingolipids are required for ZIKV replication.

Fig. 4

a Monolayers of Vero cells pretreated for 3 days with myriocin, FB1, or a vehicle control were incubated on ice for the indicated times with 100 PFUs before washing and overlay with media without the inhibitors. Plaques were counted after 3 days. Data are representative of four independent experiments. Two-way ANOVA with Tukey’s multiple-comparison test. b Huh7 cells pretreated for 3 days with myriocin, FB1, or a vehicle control were infected with ZIKV (MOI = 20). Intracellular ZIKV replication in myriocin or FB1-treated cells was measured at the timepoints shown and plotted relative to vehicle. n = 3 independent experiments. n = 4 independent experiments. c Huh7 cells treated with inhibitors as in (b) were pretreated for 24 h with the RNA polymerase inhibitor TPB, infected as before, then maintained in TPB and inhibitor/vehicle-treated media for 8 h. At 8 hpi, intracellular replication in TPB-treated cells was measured relative to non-TPB-treated cells for each condition. n = 2 independent experiments. Data are mean ± SD; n.s. not significant, *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t test. See also the Source Data file.