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. 2020 Jul 21;10:12087. doi: 10.1038/s41598-020-68983-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth of E. coli after treatment with Innolysins Ec. Cleared fractions of muralytically active engineered proteins (see Fig. 2) were screened for the ability to inhibit growth of E. coli ATCC11303. Art-175 was provided by Lysando AG²⁹ and used as a positive control. Phage T5 endolysin (T5 Lys) and receptor binding protein (Pb5) were used as negative controls. Growth inhibition was detected as the absence of growth (OD_655nm) of cells treated with an Innolysin in comparison to the negative control (growth of ATCC11303 cells treated with cleared lysate of cells carrying the empty vector, pVTD) after incubation for 18 h at 37 °C. *Significant bacterial growth inhibition at P < 0.05.