Figure 3.

Distinct mutational effects mapping onto the interface. (A) Illustration of the interfacial loops and their mutational effects, where mutations on Loops 1–3 (highlighted in lightpink) exert moderate perturbation to the active site, with the exception of F587 (highlighted in hotpink), mutations on Loops 4–5 (highlighted in black) renders the protein inactive, and mutations on other distant loops (highlighted in hotpink) yield the desired perturbation. (B) Possible secondary interaction between the disordered C-terminal tail of a2AP with the autolysis loop and 70–80 loops of mPlm, interpreting the mutational effects of these two loops.