Fig. 6.

iMSC-sEV inhibit stroke-induced autophagy partially via activation of STAT3 signaling pathway. a, b Rats were intravenously treated with vehicle (500 μl, PBS), iMSC-sEV (1 × 10¹¹ particles in 500 μL PBS), or iMSC-sEV with stattic (3.75 mg/kg) 4 h after MCAO, and infarct brain tissue from different groups was harvested 24 h after MCAO for western blotting. Sham rats were used as control. Representative images (a) and quantification (b) of the proteins including p-STAT3/STAT3, LC3-II/LC3-I, Beclin-1, and P62 in the indicated groups. N = 3–5 per group. c, d HUVECs were challenged by OGD for 8 h, followed by treatment with vehicle (PBS), iMSC-sEV (1 × 10⁹ particles/mL), or iMSC-sEV with stattic (5 μM) under normal culture condition for another 24 h. HUVECs cultured under normoxia condition without treatment were used as control. Representative bands (c) and quantification (d) of the proteins including p-STAT3/STAT3, LC3-II/LC3-I, Beclin-1, and P62 in the indicated groups. N = 3 per group. Data are presented as mean ± SD. *P < 0.05