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. 2020 Jul 22;11:311. doi: 10.1186/s13287-020-01826-0
Gene Primer Primer sequence PCR amplified product (bp)
Actin Forward 5′-AGAGGGAAATCGTGCGTGAC-3′ 195
Reverse 5′-CCATACCCAAGAAGGAAGGCT-3′
GADPH Forward 5′-TGTGTCCGTCGTGGATCTGA-3′ 150
Reverse 5′-TTGCTGTTGAAGTCGCAGGAG-3′
TGFβ1 Forward 5′-GACTCTCCACCTGCAAGACC-3′ 100
Reverse 5′-GGACTGGCGAGCCTTAGTTT-3′
Collagen I Forward 5′-GTGTTTCCTGTGCTACTG-3′ 132
Reverse 5′-TCTTTCTCCTCTCTGACC-3′
α-SMA Forward 5′-CCTCGCCTCTACCCCTTA-3′ 120
Reverse 5′-ATTCGCTTGCCTTTGCTT-3′