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. 2020 Jul 22;11:309. doi: 10.1186/s13287-020-01822-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — kECM concentration optimization via viability assay. Live/Dead staining was employed to evaluate the viability of USC-organoids in different kECM concentration. Green fluorescence indicated live cells, and red fluorescence-labeled dead cells. The red fluorescence intensity was quantified. With low kECM concentration, the 3D cell culture simulation effect was poor, and the ability to obtain renal cell phenotype was also poor. Otherwise, high kECM concentration would affect the viscosity of the medium and finally influence nutrition penetration. From the figure, the 10% kECM was the optimal concentration for organoid formation (scale bar 200 μm)