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. 2020 Jul 22;12:111. doi: 10.1186/s13148-020-00900-x

Table 2.

Summary of the studies investigating the proportions of ART-conceived livebirths in imprinting disorders

IDs Country Number of patients Number of patients with molecular analysis Non-ART ART (%) Genetic causes found in ART-conceived IDs Interventions of ART performed
Sutcliffe, 2006* [18] UK
 Total 317 12a 295 22 IVF, ICSI
  BWS 79 8 68 11 (13.9) epi, 8d; not doing genetic testing, 3
  PWS 163 2 154 9 (5.5) del, 2; not doing genetic testing, 7
  AS 75 2 73 2 (2.7) epi, 1; del, 1
Doornbos 2007* [19] Netherlands
 Total 220 11a 206 14 IVF, ICSI,
  BWS 71 6 65 6 (8.5) epi, 6d COS, IUI
  PWS 86 3 82 4 (4.7) del, 3; not doing genetic testing, 1
  AS 63 2 59 4 (6.3) del, 2; not doing genetic testing, 2
Tenorio 2016* [20] Spain
 BWS 156 156b 139 17 (10.9) epi, 15d; unknown, 2 IVF, AI, COS
Mussa, 2017* [21] Italy
 BWS 38 7a 31 7 (18.4) epi, 3d; UPD, 2; unknown, 2 IVF, ICSI, IUI
Hattori, 2019* [14] Japan
 Total 931 556 888 43 IVF, ICSI
  SRS 67 22c 59 8 (11.9) epi, 5; not doing genetic testing, 3
  BWS 117 43 110 7 (6.0) epi, 4d; not doing genetic testing, 3
  PWS 520 366 496 24 (4.6) epi, 6; del, 6; UPD, 9; not doing genetic testing, 3
  AS 227 147 223 4 (1.8) del, 4
This study** Japan
 Total 136 136b 114 22 IVF, ICSI, FET
  SRS 77 77 65 12 (15.6) epi, 12
  BWS 31 31 23 8 (25.8) epi, 8e
  KOS 5 5 5 0
  TS14 4 4 3 1 (25.0) epi, 1
  PWS 4 4 4 0
  AS 5 5 5 0
  PHP1B 8 8 7 1 (12.5) epi, 1
  TNDM 2 2 2 0

IDs imprinting disorders, ART assisted reproductive technology, BWS Beckwith-Wiedemann syndrome, PWS Prader-Willi syndrome, AS Angelman syndrome, SRS Silver-Russell syndrome, KOS Kagami-Ogata syndrome, TS14 Temple syndrome, PHP1B pseudohypoparathyroidism 1B, TNDM transient neonatal diabetes mellitus, UK United Kingdom, epi epimutation, del deletion, UPD uniparental disomy, IVF in vitro fertilization, ICSI intracytoplasmic sperm injection, COS controlled ovarian stimulation, IUI intrauterine insemination, AI artificial insemination, FET frozen embryo transfer

*Patients with all genetic causes resulting in IDs were included

**Patients with only epimutation-mediated IDs were included

aMolecular analysis was performed in patients with ART-conceived IDs only

bMolecular analysis was performed in all patients

cIncluding 10 patients from our study

dAll patients had hypomethylated KCNQ1OT1:TSS-DMR

ePatients had either hypomethylated KCNQ1OT1:TSS-DMR or hypermethylated H19/IGF2:IG-DMR