Table 3.

Concentration ranges and breakpoints of disinfectants used to determine the MIC₂₄ and MBC₂₄ values for B. cepacia ATCC 10673 and E. faecalis ATCC 29212

Tube NO	MIC24 = MBC24 (mg/L)			MIC24 = MBC24 (mg/L)
	E. faecalis ATCC 29212			B. cepacia ATCC 10673
	2% Steranios	DH	MQ	2% Steranios	DH	MQ
1	5035.00	5000.00	4500.00	5035.00	5000.00	4500.00
2	2517.50	2500.00	2250.00	2517.50	2500.00	2250.00
3	1258.75	1250.00	1125.00	1258.75	1250.00	1125.00
4	629.38	625.00	562.50	629.38	625.00	562.50
5	314.69	312.50	281.25	314.69	312.50	281.25
6	157.34	156.25	140.63	157.34	156.25	140.63
7	78.67	78.13	70.31	78.67	MIC = MBC = 78.13	MIC = MBC = 70.31
8	39.34	39.06	35.16	39.34	39.06 TO*	35.16 TO*
9	19.67	19.53	17.58	19.67	19.53	17.58
10	9.83	MIC = MBC = 9.77	8.79	MIC = MBC = 9.83	9.77	8.79
11	4.92	4.88 TO*	4.39	4.92 TO*	4.88	4.39
12	2.46	2.44	MIC = MBC = 2.20	2.46	2.44	2.20
13	1.23	1.22	1.10 TO*	1.23	1.22	1.10
14	0.61	0.61	0.55	0.61	0.61	0.55
15	MIC = MBC = 0.31	0.31	0.27	0.31	0.31	0.27
16	0.15 TO*	0.15	0.14	0.15	0.15	0.14
17	0.08	0.08	0.07	0.08	0.08	0.07
18	0.04	0.04	0.03	0.04	0.04	0.03
19	0.02	0.02	0.02	0.02	0.02	0.02
20	0.0096	0.0095	0.0086	0.0096	0.0095	0.0086
12	0.0048	0.0048	0.0043	0.0048	0.0048	0.0043
22	0.0024	0.0024	0.0021	0.0024	0.0024	0.0021
23	0.0012	0.0012	0.0011	0.0012	0.0012	0.0011
24	0.0006	0.0006	0.0005	0.0006	0.0006	0.0005
25	0.0003	0.0003	0.0003	0.0003	0.0003	0.0003
26	Negative controls (disinfectant + MHB)
27	Positive (inoculum + MHB)

*TO Turbidity was observed

*At this concentration and lower (Up to tube number 25) turbidity was observed and indicates a non-bactericidal range of disinfectants