Table 2.
Therapeutic uses of ADSC-CM
| Ref. | Author | Research in vitro | Research in vivo | Results |
|---|---|---|---|---|
| [29] | Eun Young Lee | HDFs were incubated with norCM or hypoCM | Two circular full-thickness wounds of 8 mm diameter were created on the backs of the mice. |
1. HypoCM promotes HDFs migration and type I collagen secretion and significantly reduced the size of the wound area and depth 2. The wound-healing effect of hypoCM was significantly reduced by the addition of the antibodies of both VEGF and bFGF |
| [30] | Denise R. Cooper | Co-culture of ADSC-CM with HDF | Rat model of ischemic wound healing |
1. ADSC-CM promotes HDF migration and accelerates closure of ischemic wounds. 2. There was no difference between unconditioned media or ADSC-CM for non-ischemic wounds. |
| [31] | Talita Stessuk | Co-culture of ADSC-CM and PRP with fibroblasts and keratinocytes | 1.PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds. | |
| [15] | Jiajia Zhao | Co-culture of ADSC-CM with HDF |
1. EGF, PDGF-AA, VEGF, and bFGF had high concentrations in ADSC-CM. 2. The migration of skin fibroblasts could be significantly stimulated by VEGF, bFGF, and PDGF-AA, and the proliferation could be significantly stimulated by bFGF and EGF in ASC-CM. |
|
| [32] | Min Ho Kim | The HaCaT cells and HDF were incubated with the ADSC-CM-2D or ADSC-CM-3D | 1. ADSC-CM-3D has a more significant effect on the proliferation and migration of fibroblasts and keratinocytes, the reason may be related to galectin1 expression only in 3D cultured ADSC | |
| [33] | Chengliang Deng | Fibroblast and keratinocyte were cultured in Gel-CM, SVF-CM, or serum-free medium | Full-thickness skin wound of diabetic rats |
1. Gel-CM promoted the proliferation and migration of fibroblasts & keratinocytes and increased collagen synthesis in fibroblasts 2. The wound-healing rate in the Gel-CM-treated group was significantly higher than that in the SVF-CM-treated group at all timepoints. |
| [34] | Chengliang Deng | Fibroblasts and keratinocytes were cultured in Gel-CM, Adi-CM, and SVF-CM or serum-free medium. | BALB/c nude mice wound model |
1. Gel-CM-treated group achieved complete wound healing, whereas the other groups still had unhealed wounds 2. Higher expression of bFGF, EGF, and TGF-b in Gel-CM than in other two CMs and a significantly higher expression of VEGF in Gel-CM than in SVF-CM |
| [35] | Bing-rong Zhou | Twenty-two subjects with Fitzpatrick phototypes III and IV, aged 24 to 50 |
1. ADSC-CM + FxCR can increase skin elasticity, improve skin surface roughness, and reduce transepidermal water loss, reduce pigmentation after laser 2. ADSC-CM increased dermal collagen density, elastin density, and arranged them in order. |
|
| [36] | Xi Wang | 30 female volunteers, skin type III and IV, aged 40 to 63 | 1. Microneedles + ADSC-CM can improve skin roughness, reduce melanin content, increase skin brightness, gloss, elasticity, and anti-wrinkle effects | |
| [16] | Woo-Chan Son | Co-culture of ADSC-CM with UV irradiation of HDF |
1. MMP-1 expression was significantly increased in retinoic acid-treated group and both 50 and 100% AdMSC-CM 2. Type 1 procollagen level was significantly increased in TGF-β1-treated group and both 50 and 100% AdMSC-CM treated group |
|
| [17] | Shu Guo | Co-culture of different senescent HDF with ADSC-CM before UVA irradiation |
1. ADSC-CM pretreatment was significantly reduced HDF aging rate. 2. ADSC-CM up-regulated the expression of type I, type III collagen and elastin, and downregulated the expression of MMP-1 and MMP-9 mRNA |
|
| [37] | Lu Li | HaCaTs and NHDFs were irradiated with UV and cultured with 50% and 100%ADSC-CM |
1. Both 50% and 100% ADSC-CM treatment can reduce ROS levels 2. ADSC-CM reduces the production of MMP-1 and the secretion of IL-6 by down-regulating UVB-induced MAPK and TGF-β/Smad signaling pathways 3.100% ADSC-CM treatment, the mRNA expression of procollagen type I was gradually increased in HaCaTs and NHDFs |
|
| [38] | Xiuxia Wang | Keloid fibroblasts were cultured in ADSC-CM |
1. ADSC-CM reduced the ECM-related gene expression in KFs and inhibited cell proliferation and migration 2. ADSC-CM depleted CD31+/CD34+ vessels and reduced collagen deposition |
|
| [39] | Qi Zhang | Rabbit ear hypertrophic scar model | 1. Both ADSC and ADSCs-CM treatments reduce scar hypertrophy 2. ADSCs were more effective than ADSCs-CM in reducing hypertrophic scars | |
| [40] | Yan Li | HS tissues were cultured with ADSC-CM in the presence of a p38 inhibitor and activator | BALB/c mouse excisional model |
1. ADSC-CM decreased the expression of Col1, Col3, and α-SMA in HSFs and suppressed collagen deposition in cultured HS tissues 2. ADSC-CM suppressed scar formation through the inhibition of the p38/MAPK signaling pathway in HSFs in vitro and the anti-fibrosis effect of ADSC-CM was mediated by the p38/MAPK signaling pathway in BALB/c mouse excisional models in vivo |
| [41] | Junnan Chen | HSFs were cultured in CFSC-CM or control medium |
1. CFSC-CM inhibited HSF proliferation and migration 2. CFSC-CM inhibited HSF ECM protein expression |
|
| [18] | Ji Ma | HSFs were treated with ADSCs-CM. |
1. HGF secreted by ADSC shows anti-fibrotic effect and ADSC-CM attenuates collagen production in HSFs 2. High concentrations of ADSC-CM can inhibit the Col1/Col3 ratio, reduce TIMP-1 levels, and up-regulates MMP-1 expression |
|
| [42] | Xing Shan | Adult male rabbit ears acne vulgaris scar model |
1. Almost all acne scars were cured after ADSC+CM injection in the rabbit ear acne scar model. 2. ADSC + CM reduces levels of TNF-α, IL-1α, MMP2, and keratin 16 |
|
| [43] | Peng Hao | Co-culture of ADSC-CM and glutamate-induced neurons |
1. ADSC-CM reduced glutamate-induced neuronal injury with a maximum protective effect at 50% CM and neuronal LDH release and trypsin-positive cells 2. ADSC-CM can rescue glutamate-induced neuronal energy depletion |
|
| [44] | Yu Jin Cho | HUVECs cultured in 1 ml EGM complete media supplemented ahADSC-CM | Rats MCAO model |
1. Continuous infusion of ahADSC-CM can significantly improve functional and structural recovery after stroke 2. Continuous infusion of ahADSC-CM significantly reduced the number of TUNEL-positive cells and Iba1 / TUNEL-positive cells and increased the number of CD31 + microvessels |
| [19] | Xing Wei | Hypoxic-ischemic brain injury model in neonatal rats |
1. AdSC-CM markedly attenuated both short-term and long-term effects of HI-induced brain damage and the deficit in spatial learning and memory associated with HI 2. IGF-1 and BDNF contained in ADSC-CM play an important role in the recovery of neuropathic injury and significantly reduce the long-term functional cognition and motor skills impairment of hypoxic-ischemic brain injury in rats |
|
| [45] | Hongyan Lu | HPAEC were treated with ADSC-CM | Mouse lipopolysaccharide-induced ARDS model |
1. ADSC-CM-pretreated HPAEC displayed less severe changes in response to H2O2, with attenuated gap formation and ADSC-CM treatment markedly suppressed the LPS-induced protein increase 48 h post-injection. ADSC-CM leads to lung recruitment of neutrophils with a reduced potential for oxidative response. 2. The LPS-induced level of VEGF in BALF was markedly suppressed in ADSC-CM-treated mice |
| [46] | Anandharajan Rathinasabapathy | Mouse pulmonary hypertension model and pulmonary fibrosis model |
1. ADSC-CM treatment arrest the progression of PH by improving ventricular dynamics and attenuating cardiac remodeling and improves the pulmonary vascular remodeling associated with PH 2. ADSC-CM can prevent the progression of PF in the BLEO model in a model of rat PF induced by bleomycin (BLEO) |