Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 22;11:306. doi: 10.1186/s13287-020-01814-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — METTL1 silencing accelerates embryonic body formation and increases the expression of angiogenesis-related genes. a Schematic diagram showing the EB spheroid formation process. Cells were seeded on ultralow attachment 96-well spheroid microplates to induce EB formation. b Representative images and quantification of EB formation in control and METTL1-KD hiPSCs on day 6. Scale bar, 50 μm. c qRT-PCR analysis assessing the mRNA levels in control and METTL1-KD EBs. d, e Gene ontology analysis showing functional enrichment in biological process in genes downregulated and upregulated in METTL1-KD EBs. f qRT-PCR analysis assessing the mRNA levels of angiogenesis- and vasculogenesis-related genes in control and METTL1-KD EBs. g GSEA showing enrichment of the mesoderm and cardiovascular development gene signatures. Y-axis indicates enrichment score (ES), while the X-axis indicates positively and negatively correlated gene sets. h KEGG pathway analysis of genes upregulated in METTL1-KD EBs. Data are presented as mean ± SEM; n = 3; *P < 0.05, **P < 0.01; ***P < 0.001