Fig. 1.

Schematic presentation of genome-wide methylation profiling in CMT using MBD sequencing. a Sample preparation for MBD-seq. b Sequencing data preprocessing with major parameters (window size 500 bp, filtration: bins without any CG, low signal: counts = <20, bins on Chr X). c Overall sequencing quality is visualized by IGV showing DMRs (yellow), CGI (red), and Gene information (blue). Methylation peaks are colored in 11 cancer (purple) and adjacent normal (green) samples. The region with high density of DMRs is highlighted by the red box. d High-quality signals (depth > 5X) cover more than 50% of the canine genome in 22 samples. e Analytical scheme of intergenic and genic regions or subtype-DMRs and CMT-DMRs. Additional data, CMT transcriptome and HBC expression and methylation, was investigated for further analysis