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. 2020 Jul 22;21:178. doi: 10.1186/s13059-020-02094-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — scRNA-seq analysis in egg and central cells in diploid and tetraploid plants. a A linear relationship between the total number (k = thousand) of normalized transcripts and the number of cells per library. Black dots indicate mean values with standard deviations of the total number of normalized mRNA transcripts estimated from 8 replicates for 1 cell and 5 replicates each for 2 and 3 cells. b Principal component analysis (PCA) of scRNA-seq data. ECd and ECt: egg cells in diploid and tetraploid plants, respectively; CCd and CCt: central cells in diploid and tetraploid plants, respectively. c, d Kernel density estimates of expression fold changes in the egg cells of tetraploid (ECt) and diploid (ECd) plants (c) and in the central cells of tetraploid (CCt) and diploid (CCd) plants (d)