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. 2020 Jul 15;8:742. doi: 10.3389/fbioe.2020.00742

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Copyright © 2020 Cheng, Li, Huang, Yu, Bei, Zhang, Tang, Huang and Xiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell biological activity of RHC/EGF (1:1). (A) MTT assay of NIH/3T3 cell proliferation rates on RHC, EGF, or RHC/EGF. (B) Images obtained at 0, 12, and 24 h after wound creation in vitro migration assay on RHC, EGF, or RHC/EGF. (C) Quantitative analysis of gap area of HaCaT cells cultured on RHC, EGF, or RHC/EGF. (D) Optical micrographs of crystal violet–stained NIH/3T3 cells adhering to monolayers. (E) Quantitative detection of the number of adherent NIH/3T3 cells. (F) Cytoskeleton staining. (G) Quantitative calculation of cell spread area. n = 3, means ± SD, ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 vs. control group.