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. 2020 Jun 8;7(14):2000532. doi: 10.1002/advs.202000532

Figure 6.

Figure 6

NS1 counteracts DDX21 activity in the cells. A) Coimmunoprecipitation of NS1 with WT and mutated DDX21 from cotransfected HEK293T cells. B) Immunoblotting analysis for endogenous DDX21 in HEK293T cells. C,D) Viral RNA synthesis level determination with quantitative real‐time RT‐PCR. qRT‐PCR analysis of viral RNA in wild‐type and DDX21 knock‐out HEK293T cells that transfected with indicated plasmids at C) 8 h post‐infection and D) 24 h post‐infection with WSN. *P < 0.05, **P < 0.01, ***P < 0.001, and ns, not significant (Student's t‐test, error bars, s.e.m., n = 3).