FIG 6.

DCP1A is a common target of nsp5 of different coronaviruses. (A) Alignment of the amino acid sequence of pDCP1A (GenBank accession number NP_001231287.1) with other DCP1A homologs from human (GenBank accession number NP_060873.4), cattle (GenBank accession number NP_001095800.1), mouse (GenBank accession number NP_598522.3), monkey (GenBank accession number XP_001083504.2), and dog (GenBank accession number XP_849483.2). Asterisks represent identical amino acid residues, and dots indicate similar amino acid residues. (B) HEK-293T cells cultured in 6-well plates were cotransfected with Flag-tagged pDCP1A expression plasmid and nsp5 encoded by PEDV, HCoV-229E, HCoV-NL63, HCoV-HKU1, HCoV-OC43, SARS, MERS, or PDCoV. After 28 h, cells were lysed and detected by Western blotting. (C) hDCP1A expression plasmid was transfected into HEK-293T cells with nsp5 of PEDV, HCoV-229E, HCoV-NL63, HCoV-HKU1, HCoV-OC43, SARS-CoV, MERS-CoV, or PDCoV. After 28 h, cells were lysed and detected by Western blotting.