FIG 5.
Reduced shedding in neonatal rats infected with mutant CDC-9 compared to wild-type virus. Five-day-old rat pups were inoculated with 1 × 106 FFU of the respective viruses or with clarified Vero cell culture supernatants in IMDM as a mock infection control. Rectal swabs were collected daily, and shedding was analyzed by Premier Rotaclone EIA for measuring the optical density. The data are means for 5 animals, with 3 standard errors. Differences in shedding among groups were analyzed using 2-way ANOVA with Bonferroni posttest comparison. A P value of ≤0.05 was considered significant for animals infected with CDC-9 P12 versus RRV (*), CDC-9 P12 versus Rotarix (#), and CDC-9 P12 versus CDC-9 P45 (+).