FIG 3.

More transcription is occurring per genome for strain 17syn+ than KOS(M) during acute infection of LUHMES. Shown are the relative gene expression profiles for HSV-1 ICP4 (A), TK (B), and LAT intron (C) expressed as copies per genome following a 24-h time course of infection of LUHMES with 17syn⁺ or KOS(M) at an MOI of 5. RT-qPCR was performed with primers and probes against the indicated genes (Table 2). Interestingly, on a per-genome basis, higher gene expression is seen for all three transcripts measured when LUHMES cells are infected with 17syn⁺ versus KOS(M). For each time point, significance was determined by ordinary two-way ANOVA with Sidak’s multiple-comparison test across 6 biological replicates (*, P < 0.05; ***, P < 0.0001) (n = 6).