TABLE 1.

HSV-1 strain KOS(M) is not competent for full reactivation from latency^a

Time (h) postreactivation	Cultures positive for plaques [% (no. positive/total no.)]
	17syn+	KOS(M)
0	8.3 (1/12)	0 (0/12)
3	0 (0/12)	0 (0/12)
6	50 (6/12)	0 (0/12)
12	66 (8/12)	0 (0/12)
18	16 (2/12)	0 (0/12)
24	42 (5/12)	0 (0/12)

^aThese data demonstrate a pronounced difference in phenotypic reactivation for two different strains of HSV-1. Remarkably, KOS(M) fails to produce infectious virus upon PI3K inhibitor-induced reactivation. 17syn⁺, in contrast, readily reactivates under the same conditions. We see spontaneous reactivation in latent cultures of 17syn⁺ LUHMES of around 4 to 6% (unpublished data). For these experiments, around 8% of the latent cultures (0 h) were also able to enter the lytic phase. LUHMES culture supernatants were harvested at the indicated time points following reactivation with Ly294002. The latent time point is 8 days postinfection (labeled 0 h postreactivation). Shown are the percentages of each LUHMES culture (150,000 cells) for a total population of 1.8 × 10⁶ cells that were positive for plaques during reactivation.