FIG 6.

Deletion of the 1B domain attenuates nsp10 helicase activity. (A) SDS-PAGE analysis of WT and mutants (K155A, T156A, D225A, E226A, P255A, L416A; T173A, H174A, I211A, Y320A, H321A, and Δ1B) nsp10. Molecular weight markers are shown. (B) Analysis of the ATPase hydrolysis activities of nsp10 and nsp10 Δ1B. Each protein was assayed at different concentrations (0, 0.78125, 1.5625, 3.125, 6.25, 12.5, 25, and 50 μM). (C) The dsDNA binding abilities of nsp10 and nsp10-Δ1B were determined by EMSA. Each protein was assayed at different concentrations (0, 12.5, 25, and 50 μM) with 1 μM Cy5-labeled dsDNA. (D) Analysis of the helicase activity of nsp10 Δ1B. The reaction with nsp10 WT or without protein was used are indicated as “Mock (+)” or “Mock (–),” respectively.