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. Author manuscript; available in PMC: 2020 Jul 22.
Published in final edited form as: Cell Rep. 2020 Jun 30;31(13):107839. doi: 10.1016/j.celrep.2020.107839

Figure 4. The D664A Point Mutation Also Rescues C99ΔC-Induced Dendritic Spine Loss.

Figure 4.

(A) Representative dendritic segments of hippocampal neurons in OTSCs derived from WT or APP D664A KI mice with C99ΔC as compared to control tdTomato virus. C99ΔC induced spine loss in WT cultures but not in OTSCs from APP D664A KI mice. Scale bar, 20 μm. (B) Quantification of dendritic spine densities from (A). Expression of C99ΔC induced about 50% spine loss after 24 h but was not detected in cultures from APP D664A mice and comparable to untreated control tdTomato virus-infected OTSCs. n = 15 neurons (control tdTomato) and n = 15 neurons (C99ΔC) from 10 APP D664AKI mice; n = 16 neurons (control tdTomato) and n = 18 neurons (C99ΔC) neurons from 7 WT littermates. NS, not significant; ****p ≤ 0.0001 by one-way ANOVA followed by Tukey’s multiple comparisons test.