Markers of protein synthesis and inhibitor of protein synthesis Deptor. (A) RNA content of Deptor. (B) Protein content of Akt. (C) Protein content of p-AktSer473. (D) p-AktSer473/Akt ratio. (E) p70 protein content. (F) p-p70Thr389 protein content. (G) p70/p-p70Thr389 protein content. (H) 4EBP-1 protein content. (I) p-4EBP-1Thr37/46 protein content. (J) 4EBP-1/ p-4EBP-1Thr37/46 protein content. (K) Representative images for measured Western blot targets. All graphs are means ± SEM, n = 7–10/group for each analysis. Significant trends are plotted against means with 95% confidence intervals. 1wk, 2wk, 3wk, 4wk, 1, 2, 3, and 4 weeks of cancer progression, respectively; PBS, phosphate-buffered saline.