Fig. 2.

Depletion of one Nectin homolog is insufficient to cause highly penetrant CP. (A) E14.5 Nectin1⁴⁴⁰²-transduced palatal epithelia immunostained for nectin 1 (green) and H2B-RFP reporter (red). Boxed regions show nectin 1 in greyscale. (B,C) Immunofluorescent images (B) and LUT intensity plots (C) of E14.5 Nectin4²⁵⁸⁹ and wild-type PS showing loss of nectin 4 in H2B-RFP+ cells. Bottom panels are detailed views of the boxed regions (highlighted by yellow arrows) in the top panels. Arrowheads in the bottom panels indicate RFP⁺ periderm cells. (D) Dark-field (top) and fluorescent (bottom) stereoscope images of E16.5 wild-type (left), Nectin4²⁵⁸⁹ (middle) and Nectin1⁴⁴⁰² (right) infected embryos, as in Fig. 1C. (E) Rate of CP penetrance in Nectin1 and Nectin4 knockdown embryos. (F) E16.5 palate in Nectin1⁴⁴⁰² embryo showing complete fusion. Scale bars: 4 µm in A; 25 µm in B,C; 1 mm in D; 100 µm in F.