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. 2020 Jul 13;147(21):dev189241. doi: 10.1242/dev.189241

Fig. 3.

Fig. 3.

Nectin1 and Nectin4 cooperate to promote palate closure. (A) Pooled lentiviral approach for dual Nectin1/4 knockdown. (B) Dark-field (top) and fluorescent (bottom) stereoscope images of E16.5 Nectin14402 H2B-RFP/Nectin42589 H2B-YFP infected embryo, as in Fig. 1C. Boxed region shows submucosal cleft (SMC, yellow arrow). (C) Quantification of palatal phenotypes in Nectin14402 H2B-RFP/Nectin42589 H2B-YFP embryos. (D) Double shRNA lentiviral construct for simultaneous Nectin1/Nectin4 knockdown. (E) Contrast-enhanced µCT images of E16.5 Nectin14402; Nectin42589 and littermate heads. (F,G) Quantification of palate fusion phenotypes in wild-type and Nectin14402; Nectin42589 embryos at indicated ages. (H) E15.5 µCT images of Nectin14402; Nectin42589 and littermate heads; yellow arrows indicate unilateral partially descended PS. Scale bars: 1 mm in B,E,H. *P<0.05, **P<0.01,*** P<0.001 (Fisher's exact test).