Fig. 1.

Ki67 assessment of breast cancer. A. Demarcated three scoring frames with the size of 200 × 200 μm on the hot spot. B. More than three scoring frames were demarcated when the cancer cells in three scoring frames were less than 500. C. SSSAC assessment. When clicking on a cancer cell manually the image viewing software covers the cell with a crucial marker and ordinal numbers were presented on the marker automatically. D. SSDIA assessment. The size of 25μm² was set as cut off of nucleus to separate the interstitial cells from breast cancer cells. Ki67 immunostaining of breast cancer cells was set as strong (Red), moderate (Orange), weak (yellow) and negative (Blue).