TABLE 2.
S. marcescens strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Description | Reference or source |
|---|---|---|---|
| S. marcescens strains | |||
| UMH9 | Wild type | Bloodstream infection isolate | 3 |
| Siderophore 1 (cbs) mutant | cbs::kan (Δcbs) | Full operon deletion from gene BVG96_RS08795 to BVG96_RS08815, replaced with kanamycin resistance cassette from pKD4 | This study |
| Siderophore 2 (sch) mutant | sch::kan (Δsch) | Full operon deletion from gene BVG96_RS21965 to BVG96_RS22035, replaced with kanamycin resistance cassette from pKD4 | This study |
| Siderophore 1 and siderophore 2 (cbs/sch) mutant | cbs::cam, sch::kan (Δcbs/sch) | Double mutant with both full operon deletions replaced with the indicated antibiotic resistance cassette | This study |
| Plasmids | |||
| pKD4 | Source of kanamycin resistance gene | 44 | |
| pKD3 | Source of chloramphenicol resistance gene | 44 | |
| pSIM19 | Recombineering plasmid | 45 |