TABLE 3.
Primers for mutant constructiona
| Locus | Forward primer | Reverse primer |
|---|---|---|
| Siderophore 1 (cbs) | CGCCGCTGAGGTTGCCGGCGCGGGCTCATCGTGCTCGACCAAGGCGTTCGGTGTAGGCTGGAGCTGCTTC | TGACCGAAGCGATGATGCTGCGGCCGCTGCCGTAGAAACAGGCGTACTCGATGGGAATTAGCCATGGTCC |
| Siderophore 2 (sch) | CGCTTTGGCCTCTGAATGGTTGCCGCTGGCGCCAGCGCAGCTTGATTTCTGTGTAGGCTGGAGCTGCTTC | GCGGATCACGTCGTCCGGCGCGGTGGCTTCTTTCTCGCGGCTGGAAGCCTATGGGAATTAGCCATGGTCC |
a
Primer sequences are listed 5′ to 3′. Underlined sequences in mutant construction primers are identical to regions at the 5′ to 3′ ends of the locus to be disrupted.