FIG 3.

Strain specificity of complement resistance in K. pneumoniae. Discontinuous heat map of TraDIS hits for complement resistance. Homologues across different strains were determined by BLASTp search (cutoff, >90% amino acid identity) in the process of constructing the K. pneumoniae pangenome (see Materials and Methods). Capsule and O-antigen locus types were determined using Kaptive-Web, and the corresponding gene names are used. The three genes outside the capsule and LPS loci that were required for complement resistance in all four strains are indicated in red text. Genes marked “NA” are either absent from that strain or have been excluded from the comparative analysis due to having very low read counts under any condition. Full details are in Tables S3 and S4.