FIG 4.
Salmonella-specific T cells from lymphoid organs and liver predominately express the transcription factor, T-bet, and do not express inhibitory markers. (A) Organs were harvested from infected mice infected for 40 days, and cells were stained with 2W1S-IAb-APC tetramer and surface markers. Cells were then fixed/permeabilized for transcription factor staining. Percentages of Salmonella-specific cells are shown for each transcription factor. (B) Spleens and livers were harvested from mice infected for 63 days, and cells were stained with 2W1S-IAb-APC tetramer. Cells were flow stained for each inhibitory molecule shown. Gates indicate the percentages of cell positive for each marker. Two-way ANOVA with Tukey’s post hoc analysis was used to determine statistical significance between groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. In panel A, N = 4 from two combined independent experiments. Panel B is representative of two independent experiments.