FIG 7.

Salmonella can be isolated from late chronically infected mouse livers. (A) Organs were harvested from late chronically infected mice (≥648 days postinfection) with S. Typhimurium. Organs were homogenized in 0.1% Triton X-100, then plated on LB agar plates containing 50 μg/ml streptomycin, and colonies were quantified. (B) Cultured colonies were verified by PCR by amplifying an 832-bp region specific for a conserved region of the ompC gene and a kanamycin (Kan) resistance cassette used for bacterial selection. (C) Liver sections were fixed in 4% paraformaldehyde, permeabilized, and stained with an anti-Salmonella antibody and Sytox green for nuclear counterstaining. Images were acquired at ×630 magnification using a Zeiss fluorescence microscope. Representative of 2 independent experiments.