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. 2020 Jul 9;9:e55388. doi: 10.7554/eLife.55388

Figure 1. Adiponectin signaling integrates energy metabolic state in male mice.

Figure 1.

(A) Daily profile of Adipoq mRNA expression in epididymal white adipose tissue (eWAT; n = 5 per time point). (B) Daily profile of ADIPOQ peptide in plasma (n = 3 per time point). (C) Daily profiles of Adipor1 (filled circles) and −2 (open circles) mRNA expression in the mediobasal hypothalamus (MBH; n = 5 per time point). (D, E) Daily profiles of Npy, Agrp (D), Pomc and Cartpt (E) mRNA expression in the MBH (n = 5 per time point). (F, G) Daily profiles of eWAT Adipoq mRNA expression (F) and plasma ADIPOQ levels (G) under light phase (L-RF; filled circles) or dark phase restricted feeding conditions (D-RF; open circles; n = 3 per time point). (H–J) Daily profiles of MBH Adipor1/2 (H), Npy/Agrp (I) and Pomc/Cartpt (J) mRNA expression under L-RF conditions (n = 3 per time point). (K, L) Time of day differences in eWAT Adipoq mRNA expression (K) and plasma ADIPOQ levels (L) under normal chow (NC; black) and after 1 week of high-fat diet (HFD; grey) conditions (n = 4). (M–O) Time of day differences in MBH mRNA expression of Adipor1/2 (M), Npy/Agrp (N) and Pomc/Cartpt (O) under NC and HFD conditions (n = 4). All data are means ± SEM. R – rhythmic expression (p<0.05; CircWave); N – non-rhythmic expression (p>0.05); */** - p<0.05/0.01; 2-way ANOVA with Sidak’s multiple comparisons. p values: (K) 0.005 (NC; ZT2 vs. ZT14), ANOVA dF = F(1, 14)=0.90 (interaction)/22.63 (time)/3.73 (diet); (L) 0.029 (NC; ZT2 vs. ZT14), ANOVA dF = F(1, 12)=0.45 (interaction)/15.66 (time)/14.18 (diet); (M) 0.001 (Adipor2; NC; ZT2 vs. ZT14), ANOVA dF = F(3, 24)=7.98 (interaction)/13.74 (time)/12.35 (diet); (N) 0.002 (Npy; NC; ZT2 vs. ZT14) and 0.044 (Agrp; NC; ZT2 vs. ZT14), ANOVA dF = F(3, 24)=2.51 (interaction)/32.44 (time)/8.70 (diet).

Figure 1—source data 1. Raw data of experiments shown in Figure 1.