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. 2020 Jul 9;9:e55388. doi: 10.7554/eLife.55388

Figure 2. Dampened circadian rhythms of food intake and hypothalamic clock gene expression in Adipoq-deficient male mice.

(A) Daily profiles of running-wheel activity of wild-type (WT; black) and Adipoq-deficient mice (MT; red) under 12 hr light: 12 hr dark (LD) conditions (n = 6 per group; grey shading indicates dark phase). (B) Daily food intake profiles of WT and MT mice in LD in 6 hr bins (n = 6 per group). (C) Relative light phase food intake of WT and MT mice in LD (n = 6). (D–F) circadian profiles of running-wheel activity (D; n = 12 per group), food intake (E; n = 12 per group) and relative rest phase food intake (F) of WT and MT mice in constant darkness (DD; n = 12 per group). (G–L) Circadian profiles of MBH clock gene (G–I) and metabolic gene (J–L) mRNA expression in WT and MT mice in DD (n = 3 per time point). All data are means ± SEM. R – rhythmic expression (p<0.05; CircWave); N – non-rhythmic expression (p>0.05); */**/*** - p<0.05/0.01/0.001; 2-way ANOVA with Sidak’s multiple comparisons for B, E; unpaired Student’s t-test for C, F. p-values: (B) < 0.0001 (MT vs. WT ZT12-18), ANOVA dF = F(3, 40)=8.32 (interaction)/155.4 (time)/3.03 (genotype); (C) 0.0097 (MT vs. WT); (E) 0.015 (MT vs. WT 36–42), 0.040 (42-48),<0.0001 (48-54), ANOVA dF = F(3, 88)=14.79 (interaction)/66.95 (time)/0.61 (genotype); (F) < 0.0001 (MT vs. WT).

Figure 2—source data 1. Raw data of experiments shown in Figure 2.

Figure 2.

Figure 2—figure supplement 1. Normal rest-activity rhythms in Adipoq-deficient male mice.

Figure 2—figure supplement 1.

(A) Actograms of representative male wild-type (WT; left) and Adipoq-deficient (MT; right) mice under 12 hr light: 12 hr dark (LD) and constant darkness (DD) conditions. Average DD free-running periods are depicted in the inset boxes. White-black bar on top indicates light regimen in LD. (B–D) Total running-wheel activity (B), relative light phase activity (C) and daily total food intake (D) of WT (black) and MT mice (red) in LD (n = 6 per group). (E–G) Total running-wheel activity (E), free-running period length (F) and daily total food intake (G) of WT and MT mice in DD (n = 12). All data are means ± SEM.
Figure 2—figure supplement 2. Preserved circadian clock gene expression rhythms in the SCN and peripheral tissues of Adipoq-deficient male mice.

Figure 2—figure supplement 2.

(A–I) Circadian rhythms of clock gene mRNA expression in the SCN (A–C), liver (D–F) and skeletal muscle (G–I) of male wild-type (WT; black) and Adipoq-deficient mice (MT; red) in constant darkness (DD). All data are means ± SEM (n = 3 per timepoint and genotype). R – rhythmic expression (p<0.05; CircWave); N – non-rhythmic expression (p>0.05).