Figure 4. Restoration of food intake rhythms by rhythmic activation of central adiponectin signaling in male mice.
(A) Treatment regimen sketch. Grey shading indicates dark phase. (B) Daily food intake profiles of untreated wild-type mice (WT; black; n = 9), Adipoq-deficient mice with constant AdipoRon i.c.v. infusion (MT/AR constant; red; n = 10), MT mice with rhythmic (10 hr per day) vehicle infusion (MT/Veh rhythmic; grey; n = 16), and MT mice with rhythmic AdipoRon infusion (MT/AR rhythmic; pink; n = 11). (C) Relative light phase food intake of WT mice and MT mice with constant or rhythmic AdipoRon or vehicle i.c.v infusion. (D–F) Time of day differences in MBH mRNA expression of Bmal1 (D), Npy (E) and Agrp (F) in WT and MT mice with constant (red) or rhythmic i.c.v. infusion of AdipoRon (pink) or vehicle (grey) (n = 3 per time point). All data are means ± SEM. */**/*** - p<0.05/0.01/0.001; 1-way ANOVA (C) or 2-way ANOVA with Sidak’s multiple comparisons (D–F). p-values: (C) 0.017 (MT/AR constant vs. WT), 0.007 (MT/Veh rhythmic vs. WT), ANOVA dF = F(3, 42)=6.56; (D) 0.0005 (AR rhythmic, ZT3 vs. ZT15), ANOVA dF = F(3, 16)=30.24 (interaction)/27.8 (time)/11.37 (treatment); (E) 0.0019 (WT, ZT3 vs. ZT15) and 0.0017 (AR rhythmic, ZT3 vs. ZT15), ANOVA dF = F(3, 16)=30.98 (interaction)/22.76 (time)/24.39 (treatment); (F) 0.0024 (AR rhythmic, ZT3 vs. ZT15), ANOVA dF = F(3, 16)=42.73 (interaction)/13.77 (time)/9.25 (treatment).
Figure 4—figure supplement 1. Central AdipoRon treatment has no effect on total food intake in Adipoq-deficient male mice.
