Skip to main content
. 2020 Jul 9;9:e55388. doi: 10.7554/eLife.55388

Figure 8. Rhythmic AdipoRon administration rescues food intake rhythms and body weight in obese male mice.

Figure 8.

(A) Treatment regimen and groups. (B–D) Daily food intake profiles (B), relative light phase food intake (C) and total daily food intake (D) of HFD-fed male WT mice treated 10 hr or 24 hr per day i.c.v. with AdipoRon or vehicle. (E) Body weight development of HFD-fed WT mice before and during constant or rhythmic i.c.v. AdipoRon treatment (lines indicate treatment phases). (F) Epididymal adipose tissue (eWAT) mass of HFD-fed WT mice after 3 weeks of rhythmic or constant i.c.v. treatment with AdipoRon (A-F: n = 8–11 per group). (G) Daily food intake profiles of HFD-fed male Bmal1 deficient (BKO) mice treated 10 hr or 24 hr per day i.c.v. with AdipoRon or vehicle. (H) Body weight development of HFD-fed BKO mice before and during constant or rhythmic i.c.v. AdipoRon treatment (n = 6 per group; lines indicate treatment phases). (I) eWAT mass of HFD-fed BKO mice after 3 weeks of rhythmic or constant i.c.v. treatment with AdipoRon (G-I: n = 6 per group). Shown are averages ± SEM. */**/***: p<0.05/0.01/0.001, 1-way ANOVA (C, D, F) or 2-way ANOVA with Sidak’s multiple comparisons (B, E). p-values: (B) 0.002 (WT/AR rhythmic vs. WT/Veh rhythmic; ZT0-6), ANOVA dF = F(6, 78)=4.933 (interaction)/ (61.05 (time)/0.03 (treatment); (C) 0.007 (WT/AR rhythmic vs. WT/Veh rhythmic), ANOVA dF = F(2, 26)=14.47; (E) 0.04 (WT/AR rhythmic vs. WT/Veh rhythmic; week 7), 0.001 (WT/AR rhythmic vs. WT/Veh rhythmic; week 8),<0.001 (WT/AR rhythmic vs. WT/Veh rhythmic; week 9), ANOVA dF = F(18, 234)=13.51 (interaction)/550.1 (time)/3.68 (treatment); (F) 0.033 (WT/AR rhythmic vs. WT/Veh rhythmic), ANOVA dF = F(2, 26)=4.709.

Figure 8—source data 1. Raw data of experiments shown in Figure 8.