shRNA
Target	Sense		References
scramble	CCTAAGGTTAAGTCGCCCTCG		Addgene plasmid #8455
Adipor1 KD	GTACGTCCAGGCTTCAAATAA		TRCN0000249147
Adipor2 KD	GCAGGAATTTCGTTTCATGAT		TRCN0000175771
Pgc1α KD	TAACTATGCAGACCTAGATAC		TRCN0000219080
qPCR
Target	Forward	Reverse
Eef1α	CACATCCCAGGCTGACTGT	TCGGTGGAATCCATTTTGTT
Bmal1	TGACCCTCATGGAAGGTTAGAA	CAGCCATCCTTAGCACGGT
Per2	GCCAAGTTTGTGGAGTTCCTG	CTTGCACCTTGACCAGGTAGG
Dbp	AATGACCTTTGAACCTGATCCCGCT	GCTCCAGTACTTCTCATCCTTCTGT
Rev-erbα	AGCTCAACTCCCTGGCACTTAC	CTTCTCGGAATGCATGTTGTTC
Npy	CTCCGCTCTGCGACACTAC	GGAAGGGTCTTCAAGCCTTGT
Agrp	ATGCTGACTGCAATGTTGCTG	CAGACTTAGACCTGGGAACTCT
Pomc	ATGCCGAGATTCTGCTACAGT	TCCAGCGAGAGGTCGAGTTT
Adipor1	AATGGGGCTCCTTCTGGTAAC	GCAGACCTTATACACGAACTCC
Adipor2	CCTTTCGGGCCTGTTTTAAGA	GAGTGGCAGTACACCGTGTG
Pgc1a	AACCAGTACAACAATGAGCCTG	AATGAGGGCAATCCGTCTTCA
CRISPRsgRNA
Target
Bmal1 KO	CCCACAGTCAGATTGAAAAG
Cloning
Target	Forward	Reverse	References
Npy promoter	GTCCAGGAGGTGATGAACCTATGTTCTTTATGG	GCGCCCCTGTCCCAGTTGATCCTGGC	Fick et al., 2010
ChIP
Bmal1 RORE	TTGGGCACAGCGATTGGTGG	TCCGGCGCGGGTAAACAGG
Bmal1 3'UTR	CTGTGTGCATCGGACAGTC	CGAAGCCACCATCTGAAAC