Figure 7. Post-injury administration of DP but not Pom attenuated TBI-induced markers of autophagy, as evidenced by mitigation of TBI-induced changes in mRNA and protein expression of p62 and the conversion of LC3-I to LC3-II.
(A) TBI induced a reduction in the mRNA expression of p62, resulting in (B) a decrease in p62 protein levels and, additionally, (C) an increase in the expression in the LC3-II/LC3-I ratio (an indicator for late-stage autophagy) in the cortical contusion region at 24 hr. Importantly, each of these TBI-induced changes was significantly inhibited by DP, but not Pom (0.5 mg/kg).Data are expressed as mean ± S.E.M. (n = 5 in each group). *p<0.05, ***p<0.001 vs. the Sham group. #p<0.05, ##p<0.01 vs. the TBI + Veh group.
Figure 7—source data 1. DP vs. Pom (5 hr post-TBI administration) comparable efficacy to mitigate autophagy impairments.
elife-54726-fig7-data1.xlsx (101KB, xlsx)
Figure 7—source data 2. DP vs. Pom (5 hr post-TBI administration) comparable efficacy to mitigate autophagy impairments.
elife-54726-fig7-data2.xlsx (164.1KB, xlsx)
Figure 7—source data 3. DP vs. Pom (5 hr post-TBI administration) comparable efficacy to mitigate autophagy impairments.
elife-54726-fig7-data3.xlsx (306.1KB, xlsx)