Skip to main content
. 2020 Jun 23;9:e54590. doi: 10.7554/eLife.54590

Figure 6. PF4-Cre robustly labels border-associated macrophages of the brain.

(A-C) Analysis of PF4-Cre; Rosa26Ai14 recombination in (A) LYVE1+ perivascular macrophages, (B) CD206+ perivascular macrophages, and (C) IBA1+ choroid plexus macrophages. Asterisk in A: recombination in a small cluster of microglia. (D–E) PF4-Cre; Rosa26Ai14 whole-mount immunostaining of the cerebral cortex parenchyma, pia and dura for CD206 (D) and LYVE1 (E). (F) Flow cytometry quantification of microglial recombination in PF4-Cre; Rosa26Ai14 mice. (G–H) Quantification of PF4-Cre; Rosa26Ai14 recombination in (G) CD206+ and (H) LYVE1+ perivascular, pial and dural macrophages. (I) Quantification of PF4-Cre; Rosa26Ai14 recombination in IBA1+ cells of the choroid plexus. Error bars = SE (F–H), SEM (I). Scale bars = A-E-100um.

Figure 6—source data 1. Pf4-Cre robustly labels border-associated macrophages of the brain.

Figure 6.

Figure 6—figure supplement 1. PF4-Cre recombination in non-myeloid brain parenchymal cells.

Figure 6—figure supplement 1.

PF4-Cre; Rosa26Ai14 mice consistently recombined what appears to be a subpopulation of neurons of the anterior amygdala (asterisk). Scale bar = 100 µm.