Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background Mus musculus | Cx3cr1-CreER | JAX | Stock No: 020940. MGI: 5467985. |
|
| Strain, strain background Mus musculus | Pf4-Cre | JAX | Stock No: 008535. MGI: 3764698. |
|
| Antibody-linked beads | Protein A Dynabeads | Invitrogen | Catalog No: 10001D. | |
| Chemical | Cyclohexamide | Sigma | Catalog No: C7698-1G. | |
| Molecular biology reagent | RNAse Inhibitor | New England BioLabs | Catalog No: M0314S. | |
| Molecular biology reagent | Protease Inhibitors Cocktail | Sigma | Catalog No: S8830. | |
| Antibody | Anti-CD11b-PECy7 | BD Biosciences | Rat monoclonal antibody. Catalog No: 561098. RRID:AB_2033994. | Used at 1:100. |
| Antibody | Anti-CD206 | Biorad | Rat monoclonal antibody. Catalog No: MCA2235T RRID:AB_1101333. |
Used at 1:150. |
| Antibody | Anti-CD45-APC | BD Biosciences | Rat monoclonal antibody. Catalog No: 559864. RRID:AB_398672. |
Used at 1:100. |
| Antibody | Anti-CXCL10 | R and D Systems | Goat polyclonal antibody. Catalog No: AF-466-NA. RRID:AB_2292487. |
Used at 1:300. |
| Antibody | Anti-EGFP | Abcam | Chicken polyclonal antibody. Catalog No: ab13970. RRID:AB_300798. | Used at 1:300. |
| Antibody | Anti-ERTR7 | Abcam | Rat monoclonal antibody. Catalog No: Ab51824. RRID:AB_881651. |
Used at 1:150. |
| Antibody | Anti-HA | Cell Signaling | Rabbit monoclonal antibody. Catalog No: C29F4. RRID:AB_1549585. | Used at 1:300. |
| Antibody | Anti-IBA1 | Novus | Goat polyclonal antibody. Catalog No: NB100-1028. RRID:AB_521594. | Used at 1:300. |
| Antibody | Anti-LYVE1 | Abcam | Rabbit polyclonal antibody. Catalog No: ab14917. RRID:AB_301509. | Used at 1:300. |
| Antibody | Anti-NEUN | Millipore | Mouse monoclonal antibody. Catalog No: MAB377. RRID:AB_2298772. | Used at 1:100. |
| Antibody | Anti-OLIG2 | Millipore | Rabbit polyclonal antibody. Catalog No: AB9610. RRID:AB_570666. | Used at 1:300. |
| Antibody | Anti-P2RY12 | A generous gift from Dr. David Julius. | Rabbit polyclonal antibody. | Used at 1:1000. |
| Antibody | Anti-PU.1 | Cell Signaling | Rabbit polyclonal antibody. Catalog No: 2266S. RRID:AB_10692379. |
Used at 1:300. |
| Antibody | Anti-SMA-647 | Santa Cruz Biotechnologies | Mouse monoclonal antibody. Catalog No: Sc-32251. RRID:AB_262054. |
Used at 1:150. |
| Antibody | Anti-SOX9 | R and D Systems | Goat polyclonal antibody. Catalog No: AF3075. RRID:AB_2194160. | Used at 1:300. |
| Antibody | Anti-TdTomato | Chromotek | Rat monoclonal antibody. Catalog No:5F8. RRID:AB_2336064. |
Used at 1:500. |
| Antibody | Anti-TdTomato | Promega | Rabbit polyclonal antibody. Catalog No: #632496. RRID:AB_10013483. | Used at 1:500. |
| Antibody | Anti-TMEM119 | Abcam | Rabbit monoclonal antibody. Catalog No: ab209064. RRID:AB_2800343. | Used at 1:100. |