Figure 3. daedalus modulates DR-specific climbing ability.

(A and B) Plot of the day at which fewer than 20% of flies climb in tested DGRP lines, split by genotype at the most significant locus downstream of dls on (A) AL or (B) DR. The day at which 20% or fewer surviving flies are able to climb is represented by blue dots, black bars represent mean values across all tested strains with a given genotype and diet. Significance for diet interaction p < 4E-5, FDR = 3%. (C-E) The effect of Minos element insertion on (C) lifespan and (D) climbing ability over the course of life in a w¹¹¹⁸ genetic background, and (E) the log₂ difference median lifespan and unnormalized climbing decline between mutant and controls. AL shown in red, DR in blue. Significant differences between mutant and controls are indicated by *. * = p < 0.05, ** = p < 0.005, *** = p < 0.0005. nc = no change, ns = not significant. p values shown in Data S3. N = 200 flies per condition for each mutant experiment. Data in (C-E) show collective results from three biological replicates. Error bars represent SD between replicates. Data used found in Data S3. See also Figure S3E-G, L, Figure S4A-B, E-F.