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Cofactor biogenesis of CDO and its active site. (A) Crosslink formation in wild-type ADO/CDO with C-H bond cleavage. (B) Crosslink formation in F2-Tyr ADO/CDO with C-F bond cleavage. (C) Crystal structure of uncrosslinked CDO bound with L-cysteine (CYS) and nitric oxide (NO●). Cys93 exhibits two conformations. PDB entry: 6BGF.
