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. 2020 Jul 22;10:12228. doi: 10.1038/s41598-020-68917-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic, physiological and molecular analyses of nitrate responses in 10 days old light-grown and etiolated seedlings in rice. (A) Representative image of light-grown and etiolated seedlings. (B) Mesocotyl and coleoptile length of light-grown and etiolated seedlings and data represent the average of 16 seedlings. (C) Leaf chlorophyll content. The data represents the means ± SD of three independent biological replicates. (D) Effect of nitrate doses on nitrate reductase activity in the leaves of light-grown and etiolated seedlings. (E) Relative expression level of nitrate reductase and nitrite reductase genes in the leaves of light-grown and etiolated seedlings treated with either water (control) or 120 mM nitrate for 90 min, determined by qRT-PCR. ***p value < 0.001.