Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 22;10:247. doi: 10.1038/s41398-020-00917-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a–f In vitro morphological analysis of human neurons derived from iPSCs; Control #1 (a), Control #2 (c), Case #5, clone #1 (b), Case #5, clone #4 (d). e Average number of branches on the primary neurites (Control #1, 0.514 ± 0.086, n = 72; Control #2, 0.487 ± 0.087, n = 78; Case #5, clone #1, 0.252 ± 0.040, n = 147; Case #5, clone #4, 0.209 ± 0.054, n = 67; t-test P values: Control #1 vs Case #5, clone #1, p = 0.0068; Control #1 vs Case #5, clone #4, p = 0.0034; Control #2 vs Case #5, clone #1, p = 0.0153; Control #2 vs Case #5, clone #4, p = 0.0076). f Average length of primary neurites (Control #1, 116.3 ± 4.29 μm, n = 382; Control #2, 126.2 ± 3.81 μm, n = 562; Case #5, clone #1, 59.0 ± 1.88 μm, n = 575; Case #5, clone #4, 65.7 ± 3.82 μm, n = 252; t-test P values: Control #1 vs Case #5, clone #1, p = 0.0025; Control #1 vs Case #5, clone #4, p = 0.0023; Control #2 vs Case #5, clone #1, p = 0.0002; Control #2 vs Case #5, clone #4, p < 0.0001). g Two iPS cell clones derived from healthy controls and two iPS cells derived from Case #5 were differentiated into neurons, dispersed, and cultured on a plate. Time-lapse observation was then performed every 12 h and imaged for 60 h after seeding at three concentrations (0, 1, and 10 μM) of the Rho-kinase inhibitor, Y-27632. h A graph obtained by analyzing the image data of (g) the automatic tracking with NeuroTracker in IncuCyte Zoom. Culture time was plotted on the horizontal axis, while neurite outgrowth was plotted on the vertical axis. Statistical analyses are shown in Supplementary Fig. 7b. Values indicate the mean ± S.E.M.