Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 22;10:12237. doi: 10.1038/s41598-020-69242-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

NPNT protein is expressed in mouse model systems of BC. NPNT protein is present in MMTV-PyMT transgenic mice that naturally develop BC, and human cancer cells xenografts. (a) Normal FVB mouse breast tissue (14 weeks, N = 2), breast tissue from pregnant FVB mice (12 weeks, N = 2) and fully developed BC mouse tissues (14 weeks, N = 2) were analysed for the presence of NPNT protein expression by IHC. (b) Human BC cell lines MCF-7, SK-BR-3 and BT474, were injected subcutaneously to create xenograft tumours (N = 2). Tumours were harvested, fixed, sectioned and analysed by IHC for the presence of NPNT protein. Controls: primary antibody was omitted. (c) ImmunoRatio (https://153.1.200.58:8080/immunoratio/) was used to quantify the staining intensities in the IHC stained human xenograft tumours. 15 images were recorded per tumour (n = 30), p = 0.0001. Information on the cell line’s receptor status was gathered from reference²³.