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. 2020 Jul 22;10:12209. doi: 10.1038/s41598-020-68115-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inputs in the basal forebrain to cortical CRH neurons. (A) The proportion of input neurons in different subregions of the BF. Different color represent input to CRH neurons in different cortical areas; Data shown as mean ± SEM; mPFC n = 4 mice; M1 n = 4 mice; S2 n = 4 mice; S1BF n = 6 mice; Au n = 4 mice; V1 n = 4 mice. (B) The immunofluorescent staining of cholinergic neurons in LGP. The arrows point to input neurons merged with cholinergic neurons, scale bar = 100 μm. (C) Proportion of cholinergic neurons input to cortical CRH neurons in BF. (D) A diagrammatic drawing of BF cholinergic neurons projected to cortical CRH neurons. The abbreviations of brain regions are provided in Supplementary Table S2.